<a href='https://brc.riken.jp/mus/pcr01351'>Genotyping protocol -PCR-</a> mGluR2ノックアウトマウス。コカインへの依存行動が亢進している。行動の異常がある CCE/EK.CCE [129S/SvEv-Gpi1<c>] mGluR2 (Grm2, G protein-coupled receptor, family C, group 1, member B) is widely dstributed both presynaptically and postsynaptically in varietry of neural cells. mGluR deficient mice have no behavioral abnormalities. But, a locomotor sensitization to cocaine administration was increased significantly. Moreover, significant impairment in motor coordination was detected. mGluR2 deficient mice were useful to clarify the rule in synaptic regulation of glutamatergic transmission in the neural network. RBRC01351 C（3〜6か月） Kyoto Univ. 国立大学法人京都大学 Neurobiology Research C57BL/6系統（substrain不明）で8世代、その後C57BL/6N（チャールズリバー）で12世代戻し交配を行った。 B6.129S-Grm2<tm1Nak>/NakRbrc B6.129S-Grm2<tm1Nak>/NakRbrc 中西　重忠 true herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse glutamate receptor metabotropic 2 genomic DNA C (3-6 months) 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>必ず提供承諾書を必要とする。 繁殖効率A 特になし The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 273, 645-647 (1996). Proc Natl Acad Sci USA, 102, 4170-4175 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT is required to obtain a prior written consent from the DEPOSITOR on use of the BIOLOGICAL RESOURCE. Shigetada NAKANISHI 繁殖効率A特になし Developed by Yokoi et al. (1996). A neomycin resistance cassette replaced 15.3-kb DNA fragment containing exon 2. The Grm2 deficient mice were produced by backcrossing to C57BL/6 strain for 20 times. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> C57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO C57BL/6-Grm2 tm (Nov.2011), GluR2 KO, mGluR2 KO